Fertile Ultimate®
Microfluidic Sperm Sorting Chips
Fertile Ultimate® Material Safety Data Sheet
Fertile Ultimate® Features
FERTILE Ultimate®, can be used in IVF(In vitro fertilization), IUI(Intra uterine insemination), ICSI (Intracytoplasmic sperm injection). Fertile Ultimate® provides a better process for sperm washing, swimming and gradient centrifuging, making these processes with less time and stage demanding. It also sorts sperms with best DNA and physiological quality.
FERTILE Ultimate® , does not use any stage, that harms physically alive sperms unlike centrifuge or mixing with vortex
These defined stages also creates oxidative stress on cells. Studies show that oxidative stress creates DNA fragmantation (1,2).Sperms with these damaged DNA’s cause unseccesfull IVF and IUI applications. (3).
FERTILE Ultimate® , is user friendly and has reproducible outcomesi for that reason it eliminates technician, material and solution differences and errors.
FERTILE Ultimate® , provides results withing 30 minutes with best yield. It takes away long preperation times on IVF, ICSI and IUI.
Also FERTILE Ultimate® does not require any pretreatment to the sample. This decreases the contamination risk.
When working with multiple samples, confusions cam be prevented with noting ID datas or serial numbers on the microchips surface.
Microchips can be observed with light microscobe, this can provide an efficent monitorization.
Design
Fertile Ultimate® is designed for sorting sperms which have better morphology, genetic and physiologic quality from other dead or immature sperms without using sperm damagin techniques, like centrifuge or mixing with pipette, in every assisted reproductive technology, andrology or embrylogy labratory.
FERTILE Ultimate® has an inlet, for applying the sample and an outlet for collecting processed product. Fertile Plus represented steryl and single-use to users. Also, product mimics female reproducing system with microfluiding technology for natural sperm movement.
FERTILE Ultimate® Manufactured with biocompatible materials. It is designed for to have best ergonomy working safety and user friendliness.
Performance Evaluation
Viability
Motility
After working with Fertile Ultimate®, collected sperms has more than 2 times motility compared to unprocessed semen (5).
Curvilinear Velocity
After working with Fertile Plus, collected sperms has more than 1,5 times curvilinear velocity compared to unprocessed semen (5).
After working with Fertile Ultimate®, collected sperms has more than 1,5 times straight line velocity compared to unprocessed semen (5).
After working with Fertile Ultimate®, collected sperms has more than avarage path velocity compared to unprocessed semen (5).
Sperm Morphologic Analysis
Higher percentage of sperm sorted using FERTILE Plus chips were morphologically normal than stock sperm (5)
| SPERM | Assessment Comments |
| 1 | Normal |
| 2 | Bent tail |
| 3 | Small head / Coiled tail |
| 4 | Round head |
| 5 | Amorphous head / Thick neck |
Sperm Nuclear Maturity Analysis
After working with fertile Ultimate® collected alive sperm is higher mature than stock sperm sample (5).
| SPERM | Immature |
| 1 | Immature |
| 2 | Immature |
| 3 | Immature |
| 4 | Immature |
| 5 | Immature |
Reactive Oxidative Stress Products Output
Some chemicals named Reactive Oxygen Radicals is important due to creating DNA fregmantation. Semen with high fragmentation rate is highly unsuccessful fertilizing ovari (6).
